期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 160, 期 6, 页码 601-605出版社
URBAN & FISCHER VERLAG
DOI: 10.1078/0176-1617-01046
关键词
glutamine synthetase; isoenzyme distribution; maize; nitrogen metabolism
In maize (Zea mays L.), GS(p1), the predominant GS isozyme of the developing kernel, is abundant in the pedicel and pericarp, but absent from the endosperm and embryo. Determinations of GS(p1) tissue distribution in vegetative tissues have been limited thus far to root and leaves, where the isozyme is absent. However, the promoter from the gene encoding GS(p1) has been shown to drive reporter gene expression not only in the maternal seed-associated tissues in transgenic maize plants, but also in the anthers, husks and pollen (Muhitch et al. 2002, Plant Sci 163: 865-872). Here we report chromatographic evidence that GS(p1) resides in immature tassels, dehiscing anthers, kernel glumes, ear husks, cobs and stalks of maize plants, but not in mature, shedding pollen grains. RNA blot analysis confirmed these biochemical data. In stalks, GS(p1) increased in the later stages of ear development, suggesting that it plays a role in nitrogen remobilization during grain fill.
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