期刊
GENES AND IMMUNITY
卷 4, 期 4, 页码 283-288出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.gene.6363963
关键词
LTA+250; CD14-159; TNF alpha; E. coli; S. pneumoniae; LPS
Improved understanding of how host genetic variation affects resistance to microbial pathogens could lead to better treatment and/or prevention of infectious diseases. The lymphotoxin alpha (LTA)+250 and CD14-159 polymorphisms are associated with differences in susceptibility or outcome to several infections. We stimulated peripheral blood mononuclear cells (PBMC) from 22 healthy individuals with purified lipopolysaccharide (LPS), heat-killed Escherichia coli or Streptococcus pneumoniae. TNFalpha intracellular protein levels were measured by flow cytometry and mRNA was quantitated by RT-PCR. TNFalpha mRNA levels were higher in LTA+250GG subjects after 4 h incubation with LPS compared with LTA+250AA (T test, P = 0.001). In contrast, after 8 h incubation with S. pneumoniae, there was slightly more TNFalpha mRNA in cells from LTA+250AA subjects. After 4 h incubation with LPS or E. coli, CD14-159TT subjects had higher TNFalpha mRNA levels than CD14-159CC (P = 0.05, 0.033, respectively). Neither polymorphism affected the proportion of cells expressing intracellular TNFalpha protein. This suggests that the polymorphisms affected transcription and that other regulatory mechanisms affect production of TNFalpha protein. The effect of these two polymorphisms on TNFalpha mRNA production is stimulus dependent, with opposite effects observed for Gram-positive and Gram-negative stimuli.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据