期刊
JOURNAL OF NEUROPHYSIOLOGY
卷 89, 期 6, 页码 3235-3242出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/jn.01171.2002
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资金
- NINDS NIH HHS [NS-38254] Funding Source: Medline
P2X receptors have been suggested to be expressed on the central terminals of Adelta-afferent fibers innervating dorsal horn lamina V and play a role in modulating sensory synaptic transmission. These P2X receptors have been widely thought to be P2X(2+3) receptors. However, we have recently found that P2X receptor-mediated modulation of sensory transmission in lamina V is not inhibited by trinitrophenyl-adenosine triphosphate (TNP-ATP), a potent antagonist of P2X(1), P2X(3) homomers, and P2X(2 + 3) heteromers. To provide direct evidence for the presence of TNP-ATP-resistant P2X receptors on primary afferent fibers, we examined alpha,beta-methylene-ATP (alphabetameATP)-evoked currents and their sensitivity to TNP-ATP in rat dorsal root ganglion (DRG) neurons. alphabetameATP evoked fast currents, slow currents, and mixed currents that contained both fast and slow current-components. Fast currents and fast current components in the mixed currents were both completely inhibited by 0.1 muM TNP-ATP (n = 14). Both slow currents and slow-current components in the mixed currents showed broad spectrum of sensitivity to 1 muM TNP-ATP, ranging from complete block (TNP-ATP-sensitive) to little block (TNP-ATP-resistant). TNP-ATP-resistant currents evoked by 10 muM alphabetameATP could be largely inhibited by 10 muM iso-pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid. Cells with P2X currents that were highly resistant to TNP-ATP were found to be insensitive to capsaicin. These results suggest that TNP-ATP-resistant P2X receptor subtypes are expressed on capsaicin-insensitive Abeta-afferent fibers and play a role in modulating sensory transmission to lamina V neurons.
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