期刊
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
卷 129, 期 1, 页码 23-31出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0166-6851(03)00073-2
关键词
Entamoeba invadens; genomic; sequencing; surface protein; intron; multicopy genes
资金
- NIAID NIH HHS [R01 AI46516] Funding Source: Medline
Entamoeba invadens. a parasite of reptiles, is a model for the study of encystation by the human enteric pathogen Entamoeba histolytica, because E. invadens form cysts in axenic culture. With approximately 0.5-fold sequence coverage of the genome, we were able to get insights into E. invadens gene and genome features. Overall, the E. invadens genome displays many of the features that are emerging from ongoing genome sequencing efforts in E. histolytica. At the nucleotide level the E. invadens genome has on average 60% sequence identity with that of E. histolytica. The presence of introns in E. invadens was predicted with similar consensus (GTTTGT... A/TAG) sequences to those identified in E. histolyica and Entamoeba dispar. Sequences highly repeated in the genome of E. histolytica (rRNAs, tRNAs, CXXC-rich proteins, and Leu-rich repeat proteins) were found to be highly repeated in the E. invadens genome. Numerous proteins homologous to those implicated in amoebic virulence, (Gal/GalNAc lectins, amoebapores, and cysteine proteinases) and drug resistance (p-glycoproteins) were identified. Homologs of proteins involved in cell cycle, vesicular trafficking and signal transduction were identified, which may be involved in en/excystation and cell growth of E. invadens. Finally, multiple copies of a number of E. invadens genes coding for predicted enzymes involved in core metabolism and the targets of anti-amoebic drugs were identified. (C) 2003 Elsevier Science B.V. All rights reserved.
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