期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 278, 期 23, 页码 21307-21313出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M212884200
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资金
- NEI NIH HHS [EY 12982] Funding Source: Medline
- NIGMS NIH HHS [R01 GM060019, R01 GM060019-02, R01 GM 600019, R01 GM060019-01, R01 GM060019-02S1, R01 GM060019-03S1, R01 GM060019-03] Funding Source: Medline
A subfamily of regulators of G protein signaling (RGS) proteins consisting of RGS6, -7, -9, and -11 is characterized by the presence of a unique Ggamma-like domain through which they form obligatory dimers with the G protein subunit Gbeta5 in vivo. In Caenorhabditis elegans, orthologs of Gbeta5.RGS dimers are implicated in regulating both Galpha(i) and Galpha(q) signaling, and in cell-based assays these dimers regulate Galpha(i/o)- and Galpha(q/11)-mediated pathways. However, initial studies with purified Gbeta5.RGS6 or Gbeta5.RGS7 showed that they only serve as GTPase activating proteins for Galpha(o). Pull-down assays and coimmunoprecipitation with these dimers failed to detect their binding to either Galpha(o) or Galpha(q), indicating that the interaction might require additional factors present in vivo. Here, we asked if the RGS7.Gbeta5 complex binds to Galpha(q) using fluorescence resonance energy transfer (FRET) in transiently transfected mammalian cells. RGS7, Gbeta5, and Galpha subunits were tagged with yellow variants of green fluorescent protein. First we confirmed the functional activity of the fusion proteins by co-immunoprecipitation and also their effect on signaling. Second, we again demonstrate the interaction between RGS7 and Gbeta5 using FRET. Finally, using both FRET spectroscopy on cell suspensions and microscopy of individual cells, we showed FRET between the yellow fluorescence protein-tagged RGS7.Gbeta5 complex and cyan fluorescence protein-tagged Galpha(q), indicating a direct interaction between these molecules.
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