Analytical method for the determination of the aminoglycoside gentamicin in hospital wastewater via liquid chromatography electrospray-tandem mass spectrometry

A method for the determination of gentamicin residues in hospital wastewater has been developed using kanamycin as a surrogate standard. The method consists of solid-phase extraction (SPE) and detection by ion-pair chromatography with electrospray tandem mass spectrometry (LC-ES-tandem MS). The SPE was performed on a weak cation exchanger. Filtration should be avoided in the sample preparation, otherwise a significant loss of gentamicin occurs. Chromatographic separation on a C-18-column was achieved using a ternary eluent containing methanol, water and 20 mmol l(-1) heptafluorobutyric acid solution. Mean relative recoveries of the analytes in hospital wastewater varied between 107 and 111%. The limit of quantification (LOQ) was 0.20 mug l(-1) in hospital wastewater. Gentamicin was found in native hospital wastewater in a concentration range between 0.4 and 7.6 mug l(-1). (C) 2003 Elsevier Science B.V. All rights reserved.