期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1000, 期 1-2, 页码 757-778出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(03)00512-0
关键词
column selectivity; selectivity; stationary phases, LC; alkyl-silica columns, type B; hydrophobicity; steric selectivity; hydrogen bond acidity; hydrogen bond basicity; cation-exchange behavior
Columns for reversed-phase HPLC (RP-LC) can be characterized by five, retention-related parameters: H (hydrophobicity), S (steric selectivity), A (hydrogen-bond acidity), B (hydrogen-bond basicity), and C (cation-exchange behavior). In the present study, values of the latter parameters have been measured for 92 type-B (low metals content) alkyl-silica columns and compared to column properties such as ligand length, ligand concentration, pore diameter, and the presence or absence of end-capping. With the exception of five columns of unusual design, retention factors, k, for 16 representative test compounds were correlated with values of H, S, etc., within an average +/-1.2% (1 standard deviation, SD), suggesting that all significant solute-column interactions are recognized by these five column parameters. A single-valued function F is proposed to measure differences in selectivity for any two RP-LC columns whose values of H, S, etc., are known. This allows the easy selection of columns whose selectivity is desired to be either similar to or different from a starting column, for application in either routine analysis or method development. (C) 2003 Elsevier Science B.V. All rights reserved.
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