期刊
JOURNAL OF CELL BIOLOGY
卷 161, 期 5, 页码 845-851出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200303082
关键词
cell motility; cytoskeleton; tubulin; Rho GTPases; p21-activated; kinase
类别
资金
- NIGMS NIH HHS [GM61804, R01 GM061804, GM39434, R01 GM039434] Funding Source: Medline
Actin in migrating cells is regulated by Rho GTPases. However, Rho proteins might also affect microtubules (MTs). Here, we used time-lapse microscopy of PtK1 cells to examine MT regulation downstream of Rac1. In these cells, pioneer MTs growing into leading-edge protrusions exhibited a decreased catastrophe frequency and an increased time in growth as compared with MTs further from the leading edge. Constitutively active Rac1(Q61L) promoted pioneer behavior in most MTs, whereas dominant-negative Rac1(T17N) eliminated pioneer MTs, indicating that Rac1 is a regulator of MT dynamics in vivo. Rac1(Q61L) also enhanced MT turnover through stimulation of MT retrograde flow and breakage. Inhibition of p21-activated kinases (Paks), downstream effectors of Rac1, inhibited Rac1 (Q61L)-induced MT growth and retrograde flow. In addition, Rac1 (Q61L) promoted lamellipodial actin polymerization and Pak-dependent retrograde flow. Together, these results indicate coordinated regulation of the two cytoskeletal systems in the leading edge of migrating cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据