期刊
SCIENCE
卷 300, 期 5626, 页码 1751-1755出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1082915
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资金
- NIMH NIH HHS [MH01590, MH51106] Funding Source: Medline
- NINDS NIH HHS [NS36715, R01 NS036715] Funding Source: Medline
Cerebellar long-term depression (LTD) is a model of synaptic memory that requires protein kinase C (PKC) activation and is expressed as a reduction in the number of postsynaptic alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate ( AMPA) receptors. LTD was absent in cultured cerebellar Purkinje cells from mutant mice lacking the AMPA receptor GluR2 subunit and could be rescued by transient transfection with the wild-type GluR2 subunit. Transfection with a point mutant that eliminated PKC phosphorylation of Ser(880) in the carboxyterminal PDZ ligand of GluR2 failed to restore LTD. In contrast, transfection with a point mutant that mimicked phosphorylation at Ser(880) occluded subsequent LTD. Thus, PKC phosphorylation of GluR2 Ser(880) is a critical event in the induction of cerebellar LTD.
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