期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 414, 期 2, 页码 255-260出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0003-9861(02)00750-6
关键词
calcium; iron; aging
资金
- NIA NIH HHS [R01-AG17140] Funding Source: Medline
- NIEHS NIH HHS [P30-ES01896] Funding Source: Medline
Iron and iron complexes stimulate lipid peroxidation and formation of malondialdehyde (MDA). We have studied the effects of Fe2+ and ascorbate on mitochondrial permeability transition induced by phosphate and Ca2+. iron is necessary for detectable NIDA formation, but only Ca2+ and phosphate are necessary for the induction of membrane potential loss (Deltapsi) and Ca2+ release. Keeping the iron at a constant concentration and varying the Ca2+ level changed the mitochondrial Ca2+ retention times, but not the amount of MDA formation. The antioxidant butylated hydroxytoluene at low concentrations prevented MDA formation, but not mitochondrial Ca2+ release. Preincubation of mitochondria with Fe2+ decreased Ca2+ retention time in a concentration-dependent manner and facilitated Ca2+-stimulated MDA accumulation. Thus, Ca2+ phosphate-induced mitochondrial permeability transition (MPT) can be separated mechanistically from MDA accumulation. Lipid peroxidation products do not appear to participate in the initial phase of the permeability transition, but sensitize mitochondria toward NIPT. (C) 2003 Elsevier Science (USA). All rights reserved.
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