期刊
GENERAL AND COMPARATIVE ENDOCRINOLOGY
卷 132, 期 2, 页码 248-255出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0016-6480(03)00091-1
关键词
androgen; cyproterone acetate; ELISA; hepatocytes; Oreochromis mossambicus; primary culture; tamoxifen; vitellogenin
Involvement of androgens in vitellogenin (VTG) synthesis was investigated using the primary hepatocyte cultures of tilapia, Oreochromis mossambicus. Concentration of VTG in the medium was assessed by enzyme-linked immunosorbent assay. When the hepatocytes of females were treated with testosterone (T), 17alpha-methyltestosterone (NIT) and 5alpha-dihydrotestosterone (DHT), VTG concentration in the medium slightly increased or maintained. DHT, but not T and NIT, increased VTG in the medium of male hepatocyte cultures. However, VTG production in the male hepatocytes, which were previously treated with estradiol-17beta (E-2), maintained high level by treatment of T. Similarly, co-treatment of E-2 and the androgens to the male hepatocytes enhanced VTG concentration in the medium. These results suggest that the androgens have some roles in VTG synthesis in the hepatocytes. Tamoxifen, a nonsteroidal antiestrogen, reduced VTG synthesis by the androgens. On the other hand, co-treatment of T and fadrozole, an aromatase inhibitor, failed to inhibit the effect of VTG synthesis by T alone. Analysis with RT-PCR did not demonstrate expression of the brain and the ovarian types of aromatase mRNA in the liver. These results suggest that the possibility of local aromatization of the androgens in the tilapia liver is low and that androgens bind estrogen receptor and, consequently, exert estrogenic action. Treatment of cyproterone acetate, an antiandrogen reagent, increased production of VTG with DHT. Involvement of androgens might not be ignored in regulation of VTG synthesis in the liver. (C) 2003 Elsevier Science (USA). All rights reserved.
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