期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1002, 期 1-2, 页码 93-99出版社
ELSEVIER
DOI: 10.1016/S0021-9673(03)00728-3
关键词
Morus alba; plant materials; derivatization, LC; deoxynojirimycin; alkaloids
A rapid and reliable method suitable for assays of a large number of Morus alba leaves for 1-deoxynojirimycin (DNJ) has been developed. DNJ in 0.1 g of freeze-dried leaves was double-extracted in 10 mL of aqueous 0.05 M HCl by vortexing for 15 s at room temperature, derivatized with 9-fluorenylmethyl chloroformate (FMOC-Cl), and analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC) equipped with a fluorescence detector. The double extraction recovered >99% of extractable DNJ from the leaves. Stabilization of FMOC-derivatized DNJ (DNJ-FMOC) was achieved by diluting the reactant with aqueous acetic acid after derivatization. DNJ-FMOC was stable for at least 16 days under acidic conditions at room temperature (24 degreesC). Linearity ranged between 0.3 and 30 mug mL(-1). The intra- and inter-day precision for DNJ-spiked biological samples was between 0.6 and 1.8% and between 3.7 and 4.5%, respectively. (C) 2003 Elsevier Science B.V. All rights reserved.
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