期刊
JOURNAL OF CELL BIOLOGY
卷 161, 期 6, 页码 1041-1051出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200303185
关键词
microtubule; mitosis; kinetochore; condensation; anaphase
类别
资金
- NIGMS NIH HHS [R01 GM057839, GM57839] Funding Source: Medline
Chromosome condensation is required for the physical resolution and segregation of sister chromatids during cell division, but the precise role of higher order chromatin structure in mitotic chromosome functions is unclear. Here, we address the role of the major condensation machinery, the condensin complex, in spindle assembly and function in Xenopus laevis egg extracts. Immunodepletion of condensin inhibited microtubule growth and organization around chromosomes, reducing the percentage of sperm nuclei capable of forming spindles, and causing dramatic defects in anaphase chromosome segregation. Although the motor CENP-E was recruited to kinetochores pulled poleward during anaphase, the disorganized chromosome mass was not resolved. Inhibition of condensin function during anaphase also inhibited chromosome segregation, indicating its continuous requirement. Spindle assembly around DNA-coated beads in the absence of kinetochores was also impaired upon condensin inhibition. These results support an important role for condensin in establishing chromosomal architecture necessary for proper spindle assembly and chromosome segregation.
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