期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 790, 期 1-2, 页码 365-372出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S1570-0232(03)00086-2
关键词
preparative chromatography; affinity adsorbents; Streptococcus pneumoniae; soybean; polysaccharides; bicinchoninic acid; cyan(dimethylaniino)pyridinium tetrafluoroborate; lectins; proteins; agglutinin
Optimized procedures for the affinity purification of soybean agglutinin (SBA) from soybean flour, and its further immobilization, were developed. Lectin purification on galactosyl-Sepharose yielded 44.5+/-3.5 mg of pure SBA/50 g of flour. To prepare SBA adsorbents, the lectin was immobilized onto 1-cyano-4-(dimethylamino)pyridinium tetrafluoroborate (CDAP) activated Sepharose with high yields (77%). Feasibility of the use of this improved SBA adsorbent for affinity purification of Streptococcus pneumoniae capsular polysaccharides from strain 14 (CPS-14) at laboratory scale was demonstrated. Using SBA-Sepharose adsorbent (7.0 mg lectin per ml), amounts of 6.3 mg of pure CPS-14 per cycle were produced, the adsorbent being reused up to four times without loss of capacity. (C) 2002 Elsevier Science B.V. All rights reserved.
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