期刊
MECHANISMS OF DEVELOPMENT
卷 120, 期 7, 页码 823-836出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0925-4773(03)00067-4
关键词
growth/differentiation factor 5; chondrogenesis; cell condensation; limb development; gap junction
Growth/Differentiation Factor 5 (GDF5) plays an important role in limb mesenchymal cell condensation and chondrogenesis. Here we demonstrate, using high density cultures of chick embryonic limb mesenchyme, that GDF5 misexpression increased condensation of chondroprogenitor cells and enhanced chondrogenic differentiation. These effects were observed in the absence of altered cellular viability or biosynthetic activity, suggesting that GDF5 action might be directed at the level of cellular adhesion or cell-cell communication. GDF5-enhanced condensation occurred independent of cell density or N-cadherin mediated adhesion and signaling, but was inhibited upon interference of gap junction mediated communication. p38 MAP kinase signaling was required for the GDF5 effect on chondrocyte differentiation, but not for mesenchymal condensation. These findings suggest gap junction involvement in the action of GDF5 in developmental chondrogenesis. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
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