期刊
CANCER BIOLOGY & THERAPY
卷 2, 期 4, 页码 392-395出版社
LANDES BIOSCIENCE
DOI: 10.4161/cbt.2.4.442
关键词
receptor aggregation; apoptosis; acid sphingomyelinase; ceramide; rafts
类别
资金
- NCI NIH HHS [CA 69596, CA 64268, CA 85704, CA 21765] Funding Source: Medline
- NHLBI NIH HHS [HL 6225] Funding Source: Medline
- NICHD NIH HHS [HD 35934] Funding Source: Medline
We have shown that CD95-mediated cell death requires a clustering of the receptor in distinct sphingolipid-rich domains of the cell membrane (Grassme et al., 2000, Cremesti et al., 2000). These domains form in response to acid sphingomyelinase (ASM)-induced ceramide generation. However, recent studies challenged the finding of early CD95 clustering (Algeciras-Schimnich et al., 2002). Here, six independent groups tested clustering of CD95 in diverse cell type including primary cells ex vivo and established cell lines. The studies show clustering of CD95 within seconds to minutes in all cell types tested by the different groups. In addition, clustering of CD95 was detected after stimulation of cells using three agonistic anti-CD95 antibodies (CH11, APO-1-3 and JO2), CD95 ligand and stimuli that induce an upregulation and activation of the endogenous CD95/CD95 ligand system. The data confirm our previous studies and suggest rapid, i.e., within seconds to minutes, CD95 clustering as a general phenomenon occurring in many cell types.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据