期刊
MOLECULAR REPRODUCTION AND DEVELOPMENT
卷 65, 期 3, 页码 298-308出版社
WILEY-LISS
DOI: 10.1002/mrd.10290
关键词
axoneme; flagellum; HR6B; spermatogenesis; spermatozoa; testis; Ube2
Ube2b (yeast Ubc2b/Rad6 homolog) null mice were described previously. Ube2b encodes the murine ubiquitin conjugating enzyme mHR6B. Ube2b(-/-) mice were shown to present male infertility and their sperm head shape anomalies suggested that Ube2b may be involved in the replacement of nuclear proteins during spermatid chromatin condensation. Apoptosis of spermatocytes suggested additional targets of Ube2b during spermatogenesis. Consistently, we found Ube2b transcription in both meiotic and postmeiotic stages by in situ hybridization. Immuno-electron microscopy revealed that transition proteins 1 and 2, protamines 1 and 2, and actin appear normally distributed during morphogenesis of Ube2b(-/-) spermatid heads. Surprisingly, electron microscopy revealed a particular sperm flagellum phenotype characterized by an abnormal distribution of periaxonernal structures. Flagellar anomalies of Ube2b null mice were previously described in infertile men indicating a possible genetic pathway for flagellar periaxonemal assembly in human. (C) 2003 Wiley-Liss, Inc.
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