期刊
JOURNAL OF APPLIED PHYCOLOGY
卷 15, 期 4, 页码 345-349出版社
SPRINGER
DOI: 10.1023/A:1025141913156
关键词
Gracilaria; lacZ; Malaysia; particle bombardment; seaweed; SV40 promoter; transient expression
Using a Biolistic PDS 1000/He system, healthy thalli of Gracilaria changii were bombarded with gold particles coated with plasmid DNA containing the lacZ reporter gene. Transient expression of lacZ was observed in bombarded thalli under the rupture-disc pressures of 4482, 6206, 7584 and 8963 KPa, two days after bombardment. Although G. changii exhibits a slight blue background, positive expression and the background colour can be clearly differentiated. The results indicate that lacZ could be a useful reporter gene and that SV40 promoter could be an effective promoter for Gracilaria transformation.
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