期刊
PAIN
卷 104, 期 1-2, 页码 401-413出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0304-3959(03)00048-4
关键词
descending modulation; GluR1; inflammatory hyperalgesia; rostral ventromedial medulla
资金
- NIDA NIH HHS [DA-10275] Funding Source: Medline
Our previous study demonstrated an increase in alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptor sensitivity in the rostral ventromedial medulla (RVM) associated with enhanced net descending inhibition after inflammatory hyperalgesia. The present study further studied the time-dependent changes in AMPA-produced inhibition after inflammation and the underlying molecular mechanisms. Inflammation was induced by intraplantar injection of complete Freund's adjuvant (CFA, 0.2 ml). There was a significant increase in AMPA-produced inhibition at 5 h that was further enhanced at 24 h (P < 0.05), as compared to that at 3 h post-inflammation. The AMPA-produced inhibition returned to the control level at 14 days post-inflammation. We analyzed mRNA and protein levels of the GluR1 and GluR2 AMPA receptor subunits in the RVM at 2 h to 14 days post-inflammation. AMPA receptor subunits exist in the two 'flip' and 'flop' isoforms that differentially affect the desensitization properties of the receptor. Reverse transcription-polymerase chain reaction analysis indicated that there was a significant upregulation of mRNAs encoding the GluR1-flip (5-24 h), GluR2-flip (24 h) and GIuR2-flop (24 h) isoforms in the RVM after inflammation, whereas the levels of GluR1-flop mRNAs showed no significant change. Western blots demonstrated that the GluR1 protein levels were significantly upregulated at 24 h-3 days (P < 0.05) post-inflammation, compared to that of naive animals. GluR2 protein levels remained unchanged. Immunohistochemistry further demonstrated an increase in GluR1-like immunoreactivity localized to the RVM at 24 h post-inflammation. These findings suggest that AMPA receptors in the RVM undergo selective transcriptional and translational modulation following inflammation and may contribute to activity-dependent plasticity in descending pain modulatory systems after prolonged noxious input. (C) 2003 International Association for the Study of Pain. Published by Elsevier Science B.V. All rights reserved.
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