期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 8, 期 3, 页码 381-390出版社
SPIE-INT SOCIETY OPTICAL ENGINEERING
DOI: 10.1117/1.1586704
关键词
fluorescence lifetime imaging; frequency domain; time domain; two-photon microscopy
资金
- NCRR NIH HHS [5 P41-RR03155] Funding Source: Medline
Fluorescence lifetime images are obtained with the laser scanning microscope using two methods: the time-correlated single-photon counting method and the frequency-domain method. In the same microscope system, we implement both methods. We perform a comparison of the performance of the two approaches in terms of signal-to-noise ratio (SNR) and the speed of data acquisition. While in our practical implementation the time-correlated single-photon counting technique provides a better SNR for low-intensity images, the frequency-domain method is faster and provides less distortion for bright samples. (C) 2003 Society of Photo-Optical.
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