期刊
ANALYTICA CHIMICA ACTA
卷 487, 期 1, 页码 3-14出版社
ELSEVIER
DOI: 10.1016/S0003-2670(03)00077-1
关键词
biosensors; flow injection; amperometry; laccase; phenols
Spectrographic graphite electrodes were modified through adsorption with laccase from Trametes versicolor. The laccase-modified graphite electrode was used as the working electrode in an amperometric flow-through cell for monitoring phenolic compounds in a single line flow injection system. The experimental conditions for bioelectrochemical determination of catechol were studied and optimized. The relative standard deviation of the biosensor for catechol (10 muM, n = 12) was 1.0% and the reproducibility for six laccase-modified graphite electrodes, prepared and used different days was about 11%. The optimal conditions for the biosensor operation were: 0.1 M citrate buffer solution (atpH 5.0), flow rate of 0.51 ml min(-1) and a working potential of -50 mV versus Ag\AgCl. At these conditions the responses of the biosensor for various phenolic compounds were recorded and the sensor characteristics were calculated and compared with those known for biosensors based on laccase from Coriolus hirsutus, cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium and horseradish peroxidase (HRP). (C) 2003 Elsevier Science B.V. All rights reserved.
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