期刊
JOURNAL OF FLUORESCENCE
卷 13, 期 4, 页码 323-329出版社
KLUWER ACADEMIC/PLENUM PUBL
DOI: 10.1023/A:1025377711659
关键词
excited-state proton transfer; fluorescence quenching; urea; pyranine; fluorescence decay
Excited-state proton transfer from pyranine to urea in methanol solution was studied by using both steady-state quenching and fluorescence lifetime measurements at room temperature. Proton transfer initially requires a urea monomer to form a protonated urea monomer, which is then solvated by methanol. The experimental data demonstrates the emission that would be attributed to an encounter pair. A set of rate constants is obtained on the basis of a simple kinetic model.
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