期刊
JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B
卷 21, 期 4, 页码 1472-1475出版社
A V S AMER INST PHYSICS
DOI: 10.1116/1.1582459
关键词
-
Micromechanical cantilevers were validated for the detection of antibody peptide interactions. For this purpose, gold-coated microcantilevers were chemically functionalized with an analog of the myc-tag decapeptide. Chemoselective covalent coupling of the peptide to the gold surface occurred via the sulfhydryl- group of a C-terminal cystein residue. In order to verify the surface functionalization by epifluorescence microscopy, a carboxyfluorescein reporter group was attached to the N-terminus. Binding of an anti-myc-tag antibody caused a bending of the microcantilever, resulting from a change in surface stress. The bending was recorded optically from the deflection of a laser beam. In order to compensate for bending due to nonspecific interactions, a nonfunctionalized cantilever serving as a reference was measured in parallel. A charge coupled device camera served as a position-sensitive detector, enabling the parallel and simultaneous detection of signals from an array of microcantilevers. The results validate microcantilevers for the label-free detection of antibody peptide interactions at physiological solvent conditions. (C) 2003 American Vacuum Society.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据