期刊
CLINICAL IMMUNOLOGY
卷 108, 期 1, 页码 8-20出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S1521-6616(03)00090-1
关键词
T lymphocytes; L-selectin; tumor-draining lymph nodes; adoptive immunotherapy; whole-body irradiation
类别
资金
- NCI NIH HHS [R01 CA84110] Funding Source: Medline
Activated T cells with down-regulated L-selectin expression (L-sel(-)) from tumor-draining lymph nodes represent a potent source of specific immune effectors in adoptive immunotherapy. Using congenic pairs of mice and carboxyfluorescein diacetate succinimidyl ester-labeled L-sel(-) T cells, the current study analyzed in vivo proliferation of transferred cells. In the lung of MCA205 tumor-bearing mice, 6% or 0.3 x 10(6) of the 5 x 10(6) donor cells were identified 24 h after transfer. Vigorous proliferation of donor cells was evident on day 2, reaching a maximum on day 6. The proliferation was tumor-specific and CD4 T cells divided with greater magnitude than CD8 cells. Successful adoptive immunotherapy also required sublethal whole-body irradiation (WBI) of the recipient. WBI exerted its effects on facilitating specific T cell proliferation at the tumor site. Taken together, our results demonstrate that adoptively transferred T cells undergo extensive proliferation in response to the tumor and this response is associated with therapeutic efficacy. (C) 2003 Elsevier Science (USA). All rights reserved.
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