期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 278, 期 1-2, 页码 283-292出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0022-1759(03)00231-X
关键词
monocytes; isolation; Percoll
We have optimized the procedure of monocyte isolation on a Percoll density gradient. The new procedure consists of three steps: (1) the isolation of MNC on a Ficoll density gradient; (2) the separation of monocytes from lymphocytes on a high-density hyper-osmotic Percoll density gradient; and (3) the separation of monocytes from platelets and dead cells on a low-density iso-osmotic Percoll density gradient. The procedure is simple and cost-effective. Monocyte purity and recovery are both about 75% and platelet contamination is low. The isolated monocytes retain their capacity to differentiate into dendritic cells in vitro. (C) 2003 Elsevier B.V. All rights reserved.
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