期刊
JOURNAL OF PROTEIN CHEMISTRY
卷 22, 期 5, 页码 431-439出版社
KLUWER ACADEMIC/PLENUM PUBL
DOI: 10.1023/B:JOPC.0000005458.08802.11
关键词
macromolecular crowding; GAPDH; GroEL; folding intermediate; aggregation
The effects of crowding agents, polyethylene glycol (PEG 20K), Dextran 70, and bovine serum albumin, on the denaturation of homotetrameric D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) in 0.5 M guanidine hydrochloride and the reactivation of the fully denatured enzyme have been examined quantitatively. Increasing the concentration of PEG 20K to 225 mg/ml decreases the rate constant of slow phase of GAPDH inactivation to 5% but with no change for the fast phase. Chaperone GroEL assists GAPDH refolding greatly and shows even higher efficiency under crowding condition. Crowding mainly affects refolding steps after the formation of the dimeric folding intermediate.
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