期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
卷 135, 期 3, 页码 449-460出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S1096-4959(03)00111-8
关键词
cloning; desaturase; fish; gene expression; HLTFA; lipid; nutrition; teleost
Marine fish are presumed to have a lower capacity than freshwater fish for the bioconvertion of 18C fatty acids into 20-22C highly unsaturated fatty acids (HUFA). The present work investigated the first step of this pathway, the Delta6-desaturation, in gilthead seabream. A full-length desaturase-like cDNA was identified from total RNA extracted from viscera of juvenile fish fed for 96 days on an experimental RUFA-free diet containing olive oil as the sole lipid source. The open reading frame encodes a 445-amino acid peptide that contains two membrane-spanning domains, three histidine-rich regions, and a cytochrome b(5) domain, which are characteristic of Delta6- and Delta5-desaturases. Predicted protein sequence of seabream desaturase-like indicated a high percentage of identity with mammalian Delta6-desaturases (approx. 65%). Northern analysis showed two transcripts of approximately 3.7 and 1.8 kb which were highly expressed in fish fed on HUFA-free diet and slightly expressed in fish fed on HUFA-rich diet. The fatty acid profile of the fortner group was characterized by high levels of Delta6-desaturation products (18:2 n-9 and 20:2 n-9) with no detectable levels of Delta5-desaturation product (20:3n-9). These results demonstrate for the first time the presence and nutritional modulation of a Delta6-desaturase-like cDNA in a marine fish. (C) 2003 Elsevier Science Inc. All rights reserved.
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