期刊
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
卷 14, 期 7, 页码 696-703出版社
AMER CHEMICAL SOC
DOI: 10.1016/S1044-0305(03)00204-6
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资金
- NHLBI NIH HHS [N01-HV-28179] Funding Source: Medline
A method to identify and quantify chromatin-associated proteins has been developed and applied to the analysis of changes in chromatin-associated proteins induced by Myc oncoprotein expression in human B lymphocytes. Chromatin-enriched fractions were isolated by differential detergent/salt extraction and analyzed by ICAT reagent labeling, multidimensional chromatography and tandem mass spectrometry. Many known chromatin-associated regulatory factors were identified and quantified. The method will be widely applicable to various biological systems and reveal changes in chromatin-associated regulatory factors that underlie biological phenomena. (C) 2003 American Society for Mass Spectrometry.
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