期刊
JOURNAL OF BIOCHEMISTRY
卷 134, 期 1, 页码 51-55出版社
JAPANESE BIOCHEMICAL SOC
DOI: 10.1093/jb/mvg112
关键词
pyridylamino; conversion; reducing sugar; N-linked sugar chains
Pyridylamino sugar chains were converted to the corresponding reducing sugar chains by first converting them to 1-amino-1-deoxy derivatives using the method previously reported [S. Hase, J Biochem. 112, 266-268 (1992)] and then converting the products to the corresponding reducing sugar chains using the Sommlet reaction. The reaction conditions were optimized so as to obtain the maximal product yield using 1-amino-1-deoxylactose and 1-amino-1-deoxy-N-acetylglucosamine. When the established procedure was successively applied to pyridylamino high-mannose and complex-type sugar chains, the corresponding reducing sugar chains were obtained in yields of 30%.
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