Hardenbergia virus A, a novel member of the family Betaflexiviridae from a wild legume in Southwest Australia

A 6,936-nucleotide sequence representing the complete genome of a plant virus, tentatively named Hardenbergia virus A, was obtained from Hardenbergia comptoniana. The predicted structure of the genome is a 5' untranslated region (5'UTR), a 258-kDa polyprotein in open reading frame 1 (ORF1), a 43-kDa protein in ORF2, 3'UTR and poly(A) tail. The N-terminal region of ORF1 contains a replicase protein, and the C-terminal region contains a coat protein. The protein encoded by ORF2 has homology with p30-like viral movement proteins. The predicted genome organization and phylogeny of gene products places Hardenbergia virus A within the family Betaflexiviridae.