期刊
ARCHIVES OF VIROLOGY
卷 157, 期 2, 页码 337-341出版社
SPRINGER WIEN
DOI: 10.1007/s00705-011-1162-1
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资金
- Polish Ministry of Science and Higher Education [IP 2010 012470]
- Foundation for Polish Science
Mild and necrotic isolates have been described for the European (EU) genotype of pepino mosaic virus (PepMV), an important pathogen of tomato worldwide. In this study, we produced various infectious cDNA clones of an EU isolate with point mutations introduced by site-directed mutagenesis. Our results showed that the genetic determinant responsible for necrosis induction on tomato and Datura inoxia was amino acid 67 of TGBp3. This amino acid residue also acts as necrosis determinant in PepMV isolates belonging to the Chilean 2 genotype. This demonstrates that a single point mutation plays a role in necrosis induction by PepMV, irrespective of genotype.
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