期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 54, 期 388, 页码 1675-1683出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erg184
关键词
CYP51; gene silencing; Nicotiana; obtusifoliol-14 alpha-demethylase; sterol biosynthesis
Obtusifoliol-14alpha-demethylase (CYP51) is implicated in plant sterol biosynthesis. An Arabidopsis expressed sequence tag encoding a CYP51 was used as a probe to isolate Nicotiana tabacum L. cDNAs. Two types of cDNA clones were identified. Nt CYP51-1 and Nt CYP51-2 shared 97% identity together and around 75% with other plant CYP51s. The function of the encoded enzyme has been demonstrated in planta by manipulating the sterol biosynthetic pathway at the gene level. The endogenous CYP51 of Nicotiana benthamiana was silenced upon inoculation of the plantlets with POTATO VIRUS X::Nt CYP51-1 transcripts. This resulted in the accumulation of obtusifoliol, the substrate of CYP51, and other 14alpha-methyl sterols, with a concomitant growth reduction phenotype. Virus-induced gene silencing was also applied to another steroidogenic enzyme, the Delta(7)-sterol-C5(6)-desaturase, and this resulted in the accumulation of Delta(7)-sterols in infected plants instead of the pathway end-products Delta(5)-sterols.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据