4.4 Article

DNA-binding specificity determinants of replication proteins encoded by eukaryotic ssDNA viruses are adjacent to widely separated RCR conserved motifs

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ARCHIVES OF VIROLOGY
卷 155, 期 7, 页码 1033-1046

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SPRINGER WIEN
DOI: 10.1007/s00705-010-0674-4

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  1. Instituto Potosino de Investigacion Cientifica y Tecnologica, A.C.
  2. CONACYT, Mexico [211758]
  3. Consejo Nacional de Ciencia y Tecnologia, Mexico [42639-Q, 49039]

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Eukaryotic ssDNA viruses encode a rolling-circle replication (RCR) initiation protein, Rep, which binds to iterated DNA elements functioning as essential elements for virus-specific replication. By using the iterons of all known circoviruses, nanoviruses and nanovirus-like satellites as heuristic devices, we have identified certain amino acid residues that presumably determine the DNA-binding specificity of their Rep proteins. These putative specificity determinants (SPDs) cluster in two discrete protein regions, which are adjacent to distinct conserved motifs. A comparable distribution of SPDs was uncovered in the Rep protein of geminiviruses. Modeling of the tertiary structure of diverse Rep proteins showed that SPD regions interact to form a small beta-sheet element that has been proposed to be critical for high-affinity DNA-binding of Rep. Our findings indicate that eukaryotic circular ssDNA viruses have a common ancestor and suggest that SPDs present in replication initiators from a huge variety of viral and plasmid RCR systems are associated with the same conserved motifs.

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