期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 330, 期 2, 页码 431-442出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/S0022-2836(03)00536-9
关键词
protein dynamics; protein structure; protein crystallography; peptide hormone; Raman spectroscopy
资金
- NIDDK NIH HHS [DK54622] Funding Source: Medline
- NIGMS NIH HHS [GM54072] Funding Source: Medline
The crystal structure of insulin has been investigated in a variety of dimeric and hexameric assemblies. Interest in dynamics has been stimulated by conformational variability among crystal forms and evidence suggesting that the functional monomer undergoes a conformational change on receptor binding. Here, we employ Raman spectroscopy and Raman microscopy to investigate well-defined oligomeric species: monomeric and dimeric analogs in solution, native T-6 and R-6 hexamers in solution and corresponding polycrystalline samples. Remarkably, linewidths of Raman bands associated with the polypeptide backbone (amide 1) exhibit progressive narrowing with successive self-assembly. Whereas dimerization damps fluctuations at an intermolecular P-sheet, deconvolution of the amide I band indicates that formation of hexamers stabilizes both helical and non-helical elements. Although the structure of a monomer in solution resembles a crystallographic protomer, its encagement in a native assembly damps main-chain fluctuations. Further narrowing of a beta-sheet-specific amide I band is observed on reorganization of insulin in a cross-beta fibril. Enhanced flexibility of the native insulin monomer is in accord with molecular dynamics simulations. Such conformational fluctuations may initiate formation of an amyloidogenic nucleus and enable induced fit on receptor binding. (C) 2003 Elsevier Science Ltd. All rights reserved.
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