期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 791, 期 1-2, 页码 137-147出版社
ELSEVIER
DOI: 10.1016/S1570-0232(03)00224-1
关键词
zalcitabine; lamivudine; didanosine; stavudine; zidovudine; abacavir; nevirapine
资金
- NIAID NIH HHS [9 P30 AI50410] Funding Source: Medline
- NICHD NIH HHS [HD01441] Funding Source: Medline
An accurate, sensitive and specific reversed-phase high-performance liquid chromatography assay for the simultaneous quantitative determination of the nucleoside reverse transcriptase inhibitors zalcitabine, lamivudine, didanosine, stavudine, zidovudine, and abacavir with the non-nucleoside reverse transcriptase inhibitor nevirapine in human blood plasma is described. The new Polarity X C-18 silica column used in this method provides better resolution and peak shape than all other columns tested. Also, four different ultraviolet wavelengths were used for accurate and specific quantitation of the analytes. The method was validated over the range of 10-10 000 ng/ml for all analytes except zalcitabine (10-5000 ng/ml). This method is accurate (average accuracies of three different concentrations ranged from 97.2 to 105%), and precise (within- and between-day precision measures ranged from 0.5 to 5.1% and 0.5 to 5.6%, respectively), and is currently being used for determination of plasma drug concentrations in our laboratory. (C) 2003 Elsevier Science B.V. All rights reserved.
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