期刊
JOURNAL OF CELL BIOLOGY
卷 162, 期 1, 页码 113-124出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200301006
关键词
ARF; clathrin coat assembly; PIPKI gamma; PIP2 formation; endocytosis
类别
资金
- NIDDK NIH HHS [DK 54913] Funding Source: Medline
- NINDS NIH HHS [R37 NS036251, R01 NS036251, NS 36251] Funding Source: Medline
Clathrin-mediated endocytosis of synaptic vesicle membranes involves the recruitment of clathrin and AP-2 adaptor complexes to the presynaptic plasma membrane. Phosphoinositides have been implicated in nucleating coat assembly by directly binding to several endocytotic proteins including AP-2 and AP180. Here, we show that the stimulatory effect of ATP and GTPgammaS on clathrin coat recruitment is mediated at least in part by increased levels Of PIP2. We also provide evidence for a role of ADP-ribosylation factor 6 (ARF6) via direct stimulation of a synaptically enriched phosphatidylinositol 4-phosphate 5-kinase type Igamma (PIPKIgamma), in this effect. These data suggest a model according to which activation of PIPKIgamma by ARF6-GTP facilitates clathrin-coated pit assembly at the synapse.
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