期刊
AQUATIC TOXICOLOGY
卷 64, 期 2, 页码 177-184出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0166-445X(03)00051-1
关键词
tricaine methanesulfonate; tricaine; rainbow trout; anesthesia; microsomes; biotransformation; phenol; MS-222
The effect of tricaine methanesulfonate (MS222) on rainbow trout liver biotransformation rates was investigated with a microsomal model; an in vitro preparation that can be employed with or without the use of an anaesthetic. Two experimental sets of rainbow trout microsomes were tested; one representing in vivo or surgical tricaine exposures and the other representing in vitro tissue/organ collection tricaine exposures. Microsomal incubations were performed on these two experimental groups with phenol as substrate to assess the effects of tricaine on Phase I (ring-hydroxylation) and H (glucuronidation) liver biotransformation by monitoring production of hydroquinone (HQ) catechol (CAT), and phenylglucuronide (PG). The use of a 2-h 100 mg/l exposure of tricaine for surgical anesthesia with or without 24-h recovery did not significantly (P less than or equal to 0.05) affect rates of phenol (Phase I and II) biotransformation rates; nor, did the 5-min 300 mg/l tricaine exposure for isolated organ/tissue collection significantly (P less than or equal to 0.05) affect phenol (Phase I and II) biotransformation rates. There were also no significant statistical differences (P less than or equal to 0.05) in P450 protein levels, or 7-ethoxyresorufin-O-deethylase (EROD) activity in these microsomal assays between any of the tricaine treated rainbow trout and controls. Published by Elsevier Science B.V.
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