期刊
FEBS LETTERS
卷 547, 期 1-3, 页码 228-232出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(03)00711-7
关键词
actin; intermediate filament; lens; cytoskeleton; tropomodulin
资金
- NEI NIH HHS [EY10814, F32 EY06982] Funding Source: Medline
Tropomodulin (Tmod) is an actin filament pointed end capping protein found in the membrane skeleton of lens fiber cells. We demonstrate that Tmod4 is able to bind the lens-specific intermediate filament protein, filensin, in either co-sedimentation or solid phase binding assays in a saturable fashion, but with low affinity and stoichiometry. Furthermore, Tmod4 does not bind the 53 kDa rod domain of filensin, nor to CP49, the obligate assembly partner of filensin. Finally, the binding of filensin to Tmod4 does not inhibit the actin capping activity of Tmod4 in vitro, suggesting that the two functions are not mutually exclusive. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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