期刊
JOURNAL OF EXPERIMENTAL MEDICINE
卷 198, 期 2, 页码 305-313出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20030323
关键词
Flt3/Flt3L; dendritic cells; development; hematopoietic progenitors
资金
- NCI NIH HHS [R01 CA086017, CA86017] Funding Source: Medline
- NIAID NIH HHS [R01 AI047458, R21 AI047458, AI47458] Funding Source: Medline
Stimulation of Flt3 receptor tyrosine kinase through its cognate ligand expands early hematopoietic progenitor and dendritic cells (DCs) in humans and mice. The exact developmental stages at which hematopoietic progenitors express Flt3, are responsive to its ligand, and subsequently develop to DO, are not known. Here we show that common lymphoid and common myeloid progenitors, as well as steady state DO in thymus, spleen, and epidermis, express Flt3. The receptor is down-regulated once definitive B cell, T cell, and megakaryocyte/erythrocyte commitment occurs, and Flt3 is not detectable on other steady state hematopoietic cell populations. Upon in vivo Flt3 ligand (Flt3L) administration, Flt3(+) progenitor cells and their progeny DO are expanded, whereas Flt3(-) downstream progenitors are not, or are only slightly increased. Transplantation of common lymphoid and common myeloid progenitors and subsequent Flt3L injection increases progeny DO of both precursor populations. These findings provide a definitive map of Flt3 expression in the hematopoietic hierarchy and directly demonstrate that Flt3L can drive DC development along both the lymphoid and myeloid developmental pathways from Flt3(+) progenitors to Flt3(+) DCs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据