期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 792, 期 2, 页码 363-368出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S1570-0232(03)00262-9
关键词
Achillea setacea; flavone glycosides
A simple method for the rapid separation and quantification of flavone-O- and C-glycosides in A. setacea W et K. by capillary zone electrophoresis (CZE) with UV detection is described. Using 25 mM sodium borate with 20% (v/v) of methanol (pH 9.3) as running buffer sufficient separation of the analytes was achieved within 19 min. For the quantitative determination isorhan-inetin-3-O-rutinoside was used as internal standard. The method was successfully applied to a rapid characterisation of the flavonoid complex and a precise quantification of the single and total amount of the flavonoids in different samples of A. setacea. (C) 2003 Elsevier B.V. All rights reserved.
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