期刊
CARBOHYDRATE RESEARCH
卷 338, 期 16, 页码 1691-1698出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0008-6215(03)00240-4
关键词
exopolysaccharide; (1 -> 3;1 -> 6)-beta-glucan; Botryosphaeran; Botyrosphaeria sp.; ascomyceteous fungus
The exopolysaccharide, Botryosphaeran, produced by the ligninolytic, ascomycetcous fungus Botryosphaeria sp., was isolated from the extracellular fluid by precipitation with ethanol, and purified by gel permeation chromatography to yield a carbohydrate-rich fraction (96%) composed mainly of glucose (98%). Infra-red and C-13 NMR spectroscopy showed that all the glucosidic linkages were in the beta-configuration. Data from methylation analysis and Smith degradation indicated that Botryosphaeran was a (1 --> 3)-beta-(D)-glucan with approx 22% side branching at C-6. The products obtained from partial acid hydrolysis demonstrated that the side branches consisted of single (1 --> 6)-beta-linked glucosyl, and (1 --> 6)-beta-linked gentiobiosyl residues. [GRAPHICS] (C) 2003 Elsevier Ltd. All rights reserved.
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