期刊
FEBS LETTERS
卷 548, 期 1-3, 页码 69-73出版社
WILEY
DOI: 10.1016/S0014-5793(03)00742-7
关键词
serine transport; 3-phosphoglycerate dehydrogenase; ASCT1; reverse transcriptase polymerase chain reaction
The non-essential amino acid L-serine was shown to be required to support the survival of rat cerebellar Purkinje neurons because of lack of the expression of the L-serine biosynthesis enzyme 3-phosphoglycerate dehydrogenase in them. In the present study, we investigated L-[H-3]serine uptake in primary cultures of neurons and astrocytes from the rat telencephalon. In both neurons and astrocytes, L-[H-3]serine uptake was dependent on temperature and Na+ ions, and exhibited a single component of high-affinity uptake sites (K-m = 15.0 and 17.2 muM for neurons and astrocytes, respectively). Kinetic analysis of L-[H-3]serine uptake also revealed that the uptake into neurons was faster than that into astrocytes. The selectivity of inhibition by amino acids of the L-[H-3]serine uptake resembled that of the system ASC transporters ASCT1 and ASCT2. Neutral amino acids L-alanine, L-serine, L-cysteine, and L-threonine strongly inhibited the uptake by both cell types. Furthermore, in astrocytes, but not in neurons, L-valine and L-proline also inhibited L-[H-3]serine uptake. Neither alpha-methyl aminoisobutyric acid (a system L-specific substrate) nor 2-aminobicyclo(2,2,1)heptane-2-carboxylic acid (a system L-specific substrate) inhibited the uptake of L-[H-3]serine in both neurons and astrocytes. Expression of ASCT transporters in both neurons and astrocytes was examined by use of reverse transcriptase polymerase chain reaction and immunoblot analysis. Whereas transcripts (mRNAs) of both ASCT1 and ASCT2 transporters were detected in astrocytes, only the mRNA of the former subtype was detected in neurons. Immunoblot analysis confirmed the presence of ASCT1 in both neurons and astrocytes. These findings indicate that neurons accumulate a high level of L-serine by using a Na+-dependent, high-affinity transport system, operating predominantly through the ASCT1 transporter subtype. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据