期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 23, 期 16, 页码 5502-5515出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.23.16.5502-5515.2003
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资金
- NIGMS NIH HHS [R01 GM012702, R01 GM12702, R01 GM59898, R01 GM059898] Funding Source: Medline
A nuclear mRNA degradation (DRN) system was identified from analysis of mRNA turnover rates in nup116-Delta strains of Saccharomyces cerevisiae lacking the ability to export all RNAs, including poly(A) mRNAs, at the restrictive temperature. Northern blotting, in situ hybridization, and blocking transcription with thiolutin in nup116-Delta strains revealed a rapid degradation of mRNAs in the nucleus that was suppressed by the rrp6-Delta, rail-Delta, and cbc1-Delta deletions, but not by the upfl-Delta deletion, suggesting that DRN requires Rrp6p, a 3'-to-5' nuclear exonuclease, the Rat1p, a 5'-to-3' nuclear exonuclease, and Cbc1p, a component of CBC, the nuclear cap binding complex, which may direct the mRNAs to the site of degradation. We propose that certain normal mRNAs retained in the nucleus are degraded by the DRN system, similar to degradation of transcripts with 3' end formation defects in certain mutants.
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