期刊
NUCLEIC ACIDS RESEARCH
卷 31, 期 15, 页码 4461-4471出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkg632
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资金
- FIC NIH HHS [1R03 TW1068, R03 TW001068] Funding Source: Medline
The N-6-alkyladenosines and 2-methylthio-N-6-alkyladenosines are the most common modified adenosine nucleosides and transfer ribonucleic acids (tRNA) are particularly rich in these modified nucleosides. They are present at position 37 of the anticodon arm and the contribution of these hypermodified nucleosides to codon-anticodon interactions, as well as translation, are significant, although not fully understood. Herein we described a new chemical synthesis method of the oligoribonucleotides containing N-6-alkyladenosines and 2-methylthio-N-6-alkyladenosines via post-synthetic modifications of precursor oligoribonucleotides. To obtain oligoribonucleotides containing N-6-alkyladenosines, the precursor oligoribonucleotide carrying 6-methylthiopurine riboside residue was used, whereas for the synthesis of oligoribonucleotides containing 2-methylthio-N-6-alkyladenosines the precursor oligoribonucleotide carrying the 2-methylthio-6-chloropurine riboside was applied. Among the modified oligoribonucleotides of different length and secondary structures, there were several containing naturally occurring modified nucleosides such as: N-6-isopentenyladenosine (i(6)A), N-6-methyladenosine (m(6)A), 2-methylthio-N-6-isopentenyladenosine (ms(2)i(6)A), and 2-methylthio-N-6-methyladenosine (ms(2)m(6)A), as well as several unnaturally modified adenosine derivatives.
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