期刊
JOURNAL OF NEUROBIOLOGY
卷 56, 期 2, 页码 113-124出版社
WILEY
DOI: 10.1002/neu.10220
关键词
neuromodulation; endocrine; degranulation; thalamus; median eminence
资金
- NCI NIH HHS [P30 CA013696] Funding Source: Medline
- NIDDK NIH HHS [T32 DK007328, DK07328] Funding Source: Medline
- NIMH NIH HHS [MH29380, R01 MH054088-03, MH54088, R01 MH054088, R01 MH029380-21] Funding Source: Medline
- NINDS NIH HHS [F31 NS043035, NS43035, F31 NS043035-01] Funding Source: Medline
Mast cells occur in the brain and their number changes with reproductive status. While it has been suggested that brain mast cells contain the mammalian hypothalamic form of gonadotropin-releasing hormone (GnRH-I), it is not known whether mast cells synthesize GnRH-I de novo. In the present study, mast cells in the rat thalamus were immunoreactive to antisera generated against GnRH-I and the GnRH-I associated peptide (GAP); mast cell identity was confirmed by the presence of heparin, a molecule specific to mast cells, or serotonin. To test whether mast cells synthesize GnRH-I mRNA, in situ hybridization was performed using a GnRH-I cRNA probe, and the signal was identified as being within mast cells by the binding of avidin to heparin. GnRH-I mRNA was also found, using RT-PCR, in mast cells isolated from the peritoneal cavity. Given the function of GnRH-I in the regulation of reproduction, changes in the population of brain GnRH-I mast cells were investigated. While housing males with sexually receptive females for 2 h or 5 days resulted in a significant increase in the number of brain mast cells, the proportion of mast cells positive for GnRH-I was similar to that in males housed with a familiar male. These findings represent the first report showing that mast cells synthesize GnRH-I and that the mast cell increase seen in a reproductive context is the result of a parallel increase in GnRH-I positive and non-GnRH-I positive mast cells. (C) 2003 Wiley Periodicals, Inc.
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