期刊
JOURNAL OF VIROLOGICAL METHODS
卷 111, 期 2, 页码 111-120出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0166-0934(03)00166-6
关键词
human immunodeficiency virus type 1; DNA; yeast; entry; env; chimeric
资金
- NIAID NIH HHS [AI36219, AI49170] Funding Source: Medline
- NICHD NIH HHS [N01-HD-0-3310-502-02] Funding Source: Medline
A recent shift from studies on a few subtype B laboratory human immunodeficiency virus type 1 (HIV-1) clones to analyses of extremely diverse primary HIV-1 isolates from different subtype requires the development of a rapid and generic cloning technique. This report describes the use of gap repair/recombination in yeast to shuttle env, gag, and pol genes from diverse HIV-1 subtypes into a DNA vector that can be amplified in bacteria and can express the gene of interest in mammalian cells. These diverse HIV-1 genes have also been introduced into an infectious clone to produce chimeric viruses that are useful for studies on drug susceptibility, receptor binding and fitness. (C) 2003 Elsevier B.V. All rights reserved.
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