期刊
MECHANISMS OF DEVELOPMENT
卷 120, 期 8, 页码 965-975出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0925-4773(03)00136-9
关键词
Dictyostelium discoideum; mating; cell fusion; gamete; subtraction library; EST; RNAi; Ras family protein
Macrocysts in Dictyostelium discoideum possess prototypic features of sexual reproduction and are useful for understanding the basic mechanisms of the reproductive process. Here, we randomly analyzed 1,071 gamete cDNAs, and then constructed a gamete-specific subtraction library, FC-IC. Nucleotide sequences of all 903 FC-IC clones were determined and clustered into 272 independent genes. Expression analysis based on real-time RT-PCR revealed 67 gamete-enriched genes, among which those involved in 'signal transduction' and 'multicellular organization' are prevalent. One of them, FC-IC0003, appeared also to be mating-type specific, and was named gmsA. RNAi-mediated silencing as well as disruption of gmsA reduced the cellular competency for sexual cell fusion, indicating the involvement of this gene in the sexual development of D. discoideum. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
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