期刊
PLANT AND CELL PHYSIOLOGY
卷 44, 期 8, 页码 861-867出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcg101
关键词
DNA microarray; gene transcription profiling; plastid; photosynthesis; small RNA
The plastid genome of higher plants includes about 120 genes. We adopted genomic array technologies to the tobacco plastid genome. A microarray was constructed, consisting of 220 DNA fragments that cover the whole genome sequence. Each DNA fragment corresponds to a single known gene or an intergenic region. We evaluated reliability of this microarray by comparing the plastid RNA level in light- or dark-grown tobacco seedlings. The transcripts encoding photosynthetic subunits increased significantly in light-grown tissues as expected. Furthermore, we found unexpected signals in several intergenic regions, suggesting the existence of novel transcripts in tobacco plastids.
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