期刊
PLANT JOURNAL
卷 35, 期 3, 页码 316-331出版社
WILEY
DOI: 10.1046/j.1365-313X.2003.01806.x
关键词
dicarboxylate transporter; plastid envelope membrane; photorespiration; Arabidopsis; mutant; ammonia assimilation
The Arabidopsis mutant dicarboxylate transport (dct) is one of the classic mutants in the photorespiratory pathway. It requires high CO2 levels for survival. Physiologic and biochemical characterization of dct indicated that dct is deficient in the transport of dicarboxylates across the chloroplast envelope membrane. Hence, re-assimilation of ammonia generated by the photorespiratory cycle is blocked. However, the defective gene in dct has not been identified at the molecular level. Here, we report on the molecular characterization of the defective gene in dct, on the complementation of the mutant phenotype with a wild-type cDNA, and on the functional characterization of the gene product, DiT2, in a recombinant reconstituted system. Furthermore, we provide the kinetic constants of recombinant DiT1 and DiT2, and we discuss these data with respect to their functions in ammonia assimilation. Moreover, an analysis of the transcript levels of DiT1 and DiT2 in different C-3- and C-4-type plant species is presented, and we demonstrate that the substrate specificity of DiT2 from the C-4-plant Flaveria bidentis is similar to its counterpart from C-3 plants.
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