期刊
ACS APPLIED MATERIALS & INTERFACES
卷 7, 期 4, 页码 2979-2985出版社
AMER CHEMICAL SOC
DOI: 10.1021/am508690x
关键词
microRNAs; titanium phosphate; electron transfer; electrochemical biosensor
资金
- National Basic Research Program of China [2011CB933502]
- National Natural Science Foundation of China [21475057, 21335004]
- Program for New Century Excellent Talents in University [NCET-12-0256]
MicroRNAs (miRNAs) have emerged as new candidates as diagnostic and prognostic biomarkers for the detection of a wide variety of cancers; thus, sensitive and selective detection of microRNAs is significant for early-phase cancer diagnosis and disease prevention. A novel and simple electrochemical miRNA biosensor was developed using Cd2+-modified titanium phosphate nanoparticles as signal unit, two DNA as capture probes, and Ru(NH3)(63)(+) as electron transfer mediator. Large quantities of cadmium ions were mounted in titanium phosphate spheres to output the electrochemical signal. Because of the presence of Ru(NH3)(63)(+) molecules that interacted with DNA base-pairs as electron wire, the electrochemical signal significantly increased more than 5 times. This approach achieved a wide dynamic linear range from 1.0 aM to 10.0 pM with an ultralow limit detection of 0.76 aM, exerting a substantial enhancement in sensitivity. Moreover, the proposed biosensor was sufficiently selective to discriminate the target miRNAs from homologous miRNAs and could be used for rapid and direct analysis of miRNAs in human serum. Therefore, this strategy provides a new and ultrasensitive platform for miRNA expression profiling in biomedical research and clinical diagnosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据